Halo-Trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. Coomassie and Western blot. Halo-tag antibody [28A8], monoclonal mouse IgG1and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

Halo-trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. I: Input, FT: Flow-Through, B: Bound

Chromatin Immunoprecipitation (ChIP) utilizing Halo-Trap Magnetic Agarose (otma) was performed on cross-linked chromatin isolated from the liver of a transgenic mouse line expressing a Halo-tagged version of clock factor REVERBα (HaloReverbα). Samples were isolated at timepoints of non-peak (ZT20) or peak (ZT8) binding of the REVERBα protein to the Bmal1 promoter region of the genome. The enriched DNA was then quantified by ddPCR utilizing primers directed at a gene desert (negative control region) or the Bmal1 promoter. Fold enrichment of each sample DNA is relative to that of the negative control region of each sample.

Halo-Trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. Coomassie and Western blot. Halo-tag antibody [28A8], monoclonal mouse IgG1and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

Halo-trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. I: Input, FT: Flow-Through, B: Bound

Immunoprecipitation of Halo-tag protein with Halo-Trap.I: Input, FT: Flow-through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Immunoprecipitation of Halo-tag protein with Halo-Trap.I: Input, FT: Flow-through, B: Bound.